| 112 | 0 | 111 |
| 下载次数 | 被引频次 | 阅读次数 |
贝氏柯克斯体感染母羊可引起流产，给我国养羊业带来了巨大的威胁。为了解榆林地区贝氏柯克斯体感染情况，并对其进行遗传演化分析。随机采集榆林地区6个县区334份母羊阴道拭子，利用PCR技术进行贝氏柯克斯体感染调查，对阳性样品利用分子生物学技术克隆贝氏柯克斯体omp34基因并进行序列测定，利用生物信息学进行核苷酸序列的同源性和遗传进化关系的分析。结果表明，榆林市贝氏柯克斯体的总感染率为16.77%(56/334),其中横山区感染率为61.9%(26/42)、榆阳区感染率为21.37%(25/117)、靖边县感染率为22.22%(2/9)、定边县感染率为10.71%(3/28)、绥德县和神木市未检出。基于omp34基因的遗传进化分析结果显示，分离株YY-DB和YY-YY与法国菌株(CP103435)位于同一遗传进化分支，亲缘关系最近；序列同源性分析结果显示，分离株与2022年法国菌株(CP103429、CP103431、CP103427、CP103430和CP103435)和2022年韩国菌株(CP107247和CP107268)等7株有较高的同源性，核苷酸同源性高达99.5%～99.7%。本研究揭示榆林市陕北白绒山羊感染贝氏柯克斯体较严重，提示养殖户和养殖企业需要加强Q热的防控，进一步补充了榆林地区贝氏柯克斯体的流行病学资料，也为该病防控对策的制定提供了科学依据和技术支撑。
Abstract：Coxiella burnetii(C.burnetii)infection in ewes can lead to abortion, which brings a huge threat to China's sheep farming industry.To investigate C. burnetii infection in Yulin, and further analyze its genetic evolution, 334 vaginal swabs from ewes were randomly collected from six counties/districts in the region.C.burnetii infection was detected by PCR,of which the positive samples were used to clone the omp34 gene by molecular biology techniques.Following sequencing, the homology of nucl eotide sequences and phylogenetic relationships were analyzed by bioinformatics tools.The results showed that the total infection rate of C.burnetii in Yulin City was 16.77%(56/334),of which 61.9%(26/42) was in Hengshan District, 21.37%(25/117)in Yuyang District, 22.22%(2/9)in Jingbian County, 10.71%(3/28) in Dingbian County, and no cases were detected in Suide County and Shenmu City.The results of genetic evolution analysis based on the omp34 gene indicated that the isolates YY-DB and YY-YY clustered in the same genetic and evolutionary branch as the French strain(CP103435),and the genetic relationship was the closest.Sequence homology analysis demonstrated that it had high homology with seven isolates, including French isolates in 2022(CP103429,CP103431,CP103427,CP103430,CP103435) and Korean isolates in 2022(CP107247,CP107268),with nucleotide homology ranging from 99.5% to 99.7%.This study revealed that the infection of C.burnetii was relatively severe in Shaanbei White Cashmere goats in Yulin city, which suggested that farmers and enterprises engaged in animal husbandry should strengthen Q fever prevention and control measures.These findings further supplemented the epidemiological data of C.burnetii in Yulin area and provided scientific basis and technical support for the formulation of prevention and control strategies of the disease.
[1] ULLAH Q,JAMIL T,SAQIB M,et al.Q fever—a neglectedzoonosis[J].Microorganisms,2022,10(8):1530.
[2] ZENDOIA I I,BARANDIKA J F,CEVIDANES A,et al.Coxiella burnetii infection persistence in a goat herd during seven kidding seasons after an outbreak of abortions:the effect of vaccination[J].Appl Environ Microbiol,2024,90(3):e0220123.
[3] OHLOPKOVA O V,YAKOVLEV S A,EMMANUEL K,et al.Epidemiology of zoonotic Coxiella burnetii in the Republic of Guinea[J].Microorganisms,2023,11(6):1433.
[4] JOHNSON S A M,KANEENE J B,ASARE-DOMPREH K,et al.Seroprevalence of Q fever in cattle,sheep and goats in the Volta region of Ghana[J].Vet Med Sci,2019,5(3):402-411.
[5] ESPAÑA P P,URANGA A,CILLÓNIZ C,et al.Q Fever (Coxiella Burnetii)[J].Semin Respir Crit Care Med,2020,41(4):509-521.
[6] CHIESA A,ONZA C,SULCAJ N,et al.Coxiella burnetii outbreak,Northern Italy 2021[J].One Health,2023,17:100604.
[7] SABZEVARI S,SHORAKA H,SEYYEDIN M.Seroepidemiological survey of brucellosis and Q fever among high-risk occupations in northeast of Iran for first time[J].Iran J Microbiol,2021,13(3):325-336.
[8] DHAKA P,MALIK S V S,YADAV J P,et al.Apparent prevalence and risk factors of coxiellosis (Q fever) among dairy herds in India[J].PloS one,2020,15(9):e0239260.
[9] 冯娜，苗晓茸，刘小东，等.榆林市养羊产业的发展——以陕北白绒山羊为例[J].现代畜牧兽医，2024,(09):89-92.
[10] JAHANGIRI A,RASOOLI I,OWLIA P,et al.Highly conserved exposed immunogenic peptides of omp34 against Acinetobacter baumannii:an innovative approach[J].J Microbiol Meth,2018,144:79-85.
[11] SZYMANSKA-CZERWINSKA M,GALINSKA E M,NIEMCZUK K,et al.Prevalence of Coxiella burnetii infection in foresters and ticks in south-eastern Poland and comparison of diagnostic methods[J].Ann Agric Environ Med,2013,20(4):699-704.
[12] SADIKI V,GCEBE N,MANGENA M L,et al.Prevalence and risk factors of Q fever (Coxiella burnetii) in cattle on farms of Limpopo province,South Africa[J].Front Vet Sci,2023,10:1101988.
[13] KIPTANUI J,GATHURA P B,KITALA P M,et al.Seroprevalence estimates of Q fever and the predictors for the infection in cattle,sheep,and goats in Nandi county,Kenya[J].Vet Med Int,2022,(1):3741285.
[14] 谭启东，张晓轩，周东辉，等.宁夏回族自治区奶牛感染贝氏柯克斯体的血清流行病学调查[J].中国人兽共患病学报，2017,33(04):349-352.
[15] LIU H,ZHAN Y,YI S H,et al.Molecular epidemiology of Coxiella burnetii infection in sheep and goats in Henan province,China[J].Acta Trop,2024,250:107109.
[16] ESMAEILI S,AMIRI F B,MOKHAYERI H,et al.Seroepidemiological study of Q fever,brucellosis and tularemia in butchers and slaughterhouses workers in Lorestan,western of Iran[J].Comp Immunol,Microbiol Infect Dis,2019,66:101322.
基本信息:
DOI：10.16437/j.cnki.1007-5038.2026.04.012
中图分类号:S858.27
引用信息:
[1]王欣,李荣荣,刘王叶,等.榆林市陕北白绒山羊贝氏柯克斯体感染调查及其遗传进化分析[J].动物医学进展,2026,47(04):13-18.DOI:10.16437/j.cnki.1007-5038.2026.04.012.
基金信息:
陕西省科技厅中央引导地方科技发展资金项目(2025ZY-XCZXJX-12); 国家自然科学基金地区科学基金(32060810,31960710); 陕西省创新能力支撑计划项目(2021PT-017); 榆林学院博士科研启动基金(18GK06,18GK09);榆林学院研究生创新基金项目(2024YLYCX06); 榆林市科技项目(2023-NY-15)
2026-04-15
2026-04-15